|
KMID : 0355420140380030148
|
|
Journal of Korean Academy of Oral Health
2014 Volume.38 No. 3 p.148 ~ p.153
|
|
|
Evaluation of the safety of non-thermal atmospheric-pressure plasma in hairless mouse tissues
|
|
Park Sang-Rye
Kim Gyoo-Cheon
Choi Byul-Bo-Ra
Kim Ji-Young
|
|
|
Abstract
|
|
|
Objectives: The aim of the present study was to evaluate the stability of non-thermal atmospheric-pressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues.
Methods: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment us-ing an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using he-matoxylin and eosin (H&E) and Masson’s trichrome stains.
Results: The scanning electron microscopic images revealed the morphological changes in the mem-brane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin.
Conclusions: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.
|
|
|
KEYWORD
|
|
|
Candida albicans, Hematoxylin and eosin stain, Masson’s trichrome stain, Non-thermal atmospheric pressure plasma, Scanning electron microscopy
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|